Review



phelper plasmid  (TaKaRa)


Bioz Verified Symbol TaKaRa is a verified supplier
Bioz Manufacturer Symbol TaKaRa manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    TaKaRa phelper plasmid
    Phelper Plasmid, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phelper+plasmid/AAVpro+Packaging+Plasmid/us12600969-701-1-6
    Average 94 stars, based on 40 article reviews
    phelper plasmid - by Bioz Stars, 2026-10
    94/100 stars

    Images

    Related Articles

    Transfection:

    Article Title: Graded optogenetic activation of the auditory pathway for hearing restoration.
    Article Snippet: .. In brief, triple transfection of HEK-293T cells was performed using the pHelper plasmid (TaKaRa, USA), the trans-plasmid with PHP.B capsid (Ben Deverman, Viviana Gradinaru, Caltech, USA) and the cis-plasmid with Chronos-ES/TS under the control of the human synapsin promotor. ..

    Article Title: Channelrhodopsin fluorescent tag replacement for clinical translation of optogenetic hearing restoration
    Article Snippet: .. In brief, triple transfection of HEK293T cells was performed using the pHelper plasmid (TaKaRa/Clontech), the trans -plasmid providing viral capsid AAV2/9 and the cis -plasmid providing f-Chrimson-TS Kir2.1 , f-Chrimson-TS Kir2.1 -P2A-Katushka, f-Chrimson-EYFP, or f-Chrimson, respectively. ..

    Article Title: SEMA7A orchestrates the colorectal cancer tumor microenvironment via PlexinC1: coordinating angiogenesis and macrophage M2 polarization.
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Article Title: Cerebrospinal fluid-contacting neuron tracing reveals structural and functional connectivity for locomotion in the mouse spinal cord
    Article Snippet: .. They were transfected with pAAV plasmid, pAAV2/1 (Penn vector core) or rAAV2- retro helper (Addgene, #81070 Tervo et al., 2016), and pHelper plasmid (TAKARA). ..

    Article Title: Wnt5a-Ror2 signaling activates p62-Nrf2 axis in reactive astrocytes after brain injury
    Article Snippet: AAV-PHP.eB viruses were packaged in AAVpro 293T cells (Clontech). .. Briefly, AAVpro 293T cells were cultured in DMEM (FUJIFILM Wako Pure Chemical) supplemented with 10% (v/v) FBS (NICHIREI) and transfected with the pUCmini-iCAP-PHP.eB plasmid (Addgene), pHelper plasmid (Takara Bio) and AAV transfer plasmids using TransIT-VirusGEN Transfection reagent (Mirus). ..

    Article Title: Cerebrospinal fluid-contacting neuron tracing reveals structural and functional connectivity for locomotion in the mouse spinal cord
    Article Snippet: .. They were transfected with pAAV plasmid, pAAV2/1 (Penn vector core) or rAAV2-retro helper (Addgene, #81070 ), and pHelper plasmid (TAKARA). ..

    Article Title: Retinal Epithelial Neutralization Assay Optimizes AAV Serotype Selection for Ocular Gene Therapy
    Article Snippet: .. Additionally, AAV vectors were generated via triple plasmid transfection of 293T cells, wherein the pHelper plasmid (Takara, Dalian, China, 6230), pRep-rCap plasmid (pRep-Cap2: Takara, Dalian, China, 6230; pRep-Cap5: HonorGene, Beijing, China, HG-VXH1155; pRep-Cap8: HonorGene, Beijing, China, HG-VXH1156; pRep-Cap9: HonorGene, Beijing, China, HG-VXH1157), and ITR-containing plasmid were transfected at a molar ratio of 2:1:1 using PEI. ..

    Plasmid Preparation:

    Article Title: Graded optogenetic activation of the auditory pathway for hearing restoration.
    Article Snippet: .. In brief, triple transfection of HEK-293T cells was performed using the pHelper plasmid (TaKaRa, USA), the trans-plasmid with PHP.B capsid (Ben Deverman, Viviana Gradinaru, Caltech, USA) and the cis-plasmid with Chronos-ES/TS under the control of the human synapsin promotor. ..

    Article Title: Channelrhodopsin fluorescent tag replacement for clinical translation of optogenetic hearing restoration
    Article Snippet: .. In brief, triple transfection of HEK293T cells was performed using the pHelper plasmid (TaKaRa/Clontech), the trans -plasmid providing viral capsid AAV2/9 and the cis -plasmid providing f-Chrimson-TS Kir2.1 , f-Chrimson-TS Kir2.1 -P2A-Katushka, f-Chrimson-EYFP, or f-Chrimson, respectively. ..

    Article Title: SEMA7A orchestrates the colorectal cancer tumor microenvironment via PlexinC1: coordinating angiogenesis and macrophage M2 polarization.
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Article Title: Cerebrospinal fluid-contacting neuron tracing reveals structural and functional connectivity for locomotion in the mouse spinal cord
    Article Snippet: .. They were transfected with pAAV plasmid, pAAV2/1 (Penn vector core) or rAAV2- retro helper (Addgene, #81070 Tervo et al., 2016), and pHelper plasmid (TAKARA). ..

    Article Title: Wnt5a-Ror2 signaling activates p62-Nrf2 axis in reactive astrocytes after brain injury
    Article Snippet: AAV-PHP.eB viruses were packaged in AAVpro 293T cells (Clontech). .. Briefly, AAVpro 293T cells were cultured in DMEM (FUJIFILM Wako Pure Chemical) supplemented with 10% (v/v) FBS (NICHIREI) and transfected with the pUCmini-iCAP-PHP.eB plasmid (Addgene), pHelper plasmid (Takara Bio) and AAV transfer plasmids using TransIT-VirusGEN Transfection reagent (Mirus). ..

    Article Title: Cerebrospinal fluid-contacting neuron tracing reveals structural and functional connectivity for locomotion in the mouse spinal cord
    Article Snippet: .. They were transfected with pAAV plasmid, pAAV2/1 (Penn vector core) or rAAV2-retro helper (Addgene, #81070 ), and pHelper plasmid (TAKARA). ..

    Article Title: Retinal Epithelial Neutralization Assay Optimizes AAV Serotype Selection for Ocular Gene Therapy
    Article Snippet: .. Additionally, AAV vectors were generated via triple plasmid transfection of 293T cells, wherein the pHelper plasmid (Takara, Dalian, China, 6230), pRep-rCap plasmid (pRep-Cap2: Takara, Dalian, China, 6230; pRep-Cap5: HonorGene, Beijing, China, HG-VXH1155; pRep-Cap8: HonorGene, Beijing, China, HG-VXH1156; pRep-Cap9: HonorGene, Beijing, China, HG-VXH1157), and ITR-containing plasmid were transfected at a molar ratio of 2:1:1 using PEI. ..

    Article Title: Expression control using a regulatable intron
    Article Snippet: .. The pHelper plasmid is available from Takara/Clonetech (catalogue #6234 of the AAVpro system). .. Suitable AAV2 expression systems for inducing AAV expression are widely commercially available, for example from Takara (Clonetech) as the “AAVpro Helper Free System (AAV2)”—see http://www.clontech.com/US/Products/Viral_Transduction/AAVVectorSystems/Helper_Free_Expression_System.

    Control:

    Article Title: Graded optogenetic activation of the auditory pathway for hearing restoration.
    Article Snippet: .. In brief, triple transfection of HEK-293T cells was performed using the pHelper plasmid (TaKaRa, USA), the trans-plasmid with PHP.B capsid (Ben Deverman, Viviana Gradinaru, Caltech, USA) and the cis-plasmid with Chronos-ES/TS under the control of the human synapsin promotor. ..

    Article Title: SEMA7A orchestrates the colorectal cancer tumor microenvironment via PlexinC1: coordinating angiogenesis and macrophage M2 polarization.
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Recombinant:

    Article Title: SEMA7A orchestrates the colorectal cancer tumor microenvironment via PlexinC1: coordinating angiogenesis and macrophage M2 polarization.
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Virus:

    Article Title: SEMA7A orchestrates the colorectal cancer tumor microenvironment via PlexinC1: coordinating angiogenesis and macrophage M2 polarization.
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Bioprocessing:

    Article Title: SEMA7A orchestrates the colorectal cancer tumor microenvironment via PlexinC1: coordinating angiogenesis and macrophage M2 polarization.
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Article Title: Wnt5a-Ror2 signaling activates p62-Nrf2 axis in reactive astrocytes after brain injury
    Article Snippet: AAV-PHP.eB viruses were packaged in AAVpro 293T cells (Clontech). .. Briefly, AAVpro 293T cells were cultured in DMEM (FUJIFILM Wako Pure Chemical) supplemented with 10% (v/v) FBS (NICHIREI) and transfected with the pUCmini-iCAP-PHP.eB plasmid (Addgene), pHelper plasmid (Takara Bio) and AAV transfer plasmids using TransIT-VirusGEN Transfection reagent (Mirus). ..

    Article Title: Retinal Epithelial Neutralization Assay Optimizes AAV Serotype Selection for Ocular Gene Therapy
    Article Snippet: .. Additionally, AAV vectors were generated via triple plasmid transfection of 293T cells, wherein the pHelper plasmid (Takara, Dalian, China, 6230), pRep-rCap plasmid (pRep-Cap2: Takara, Dalian, China, 6230; pRep-Cap5: HonorGene, Beijing, China, HG-VXH1155; pRep-Cap8: HonorGene, Beijing, China, HG-VXH1156; pRep-Cap9: HonorGene, Beijing, China, HG-VXH1157), and ITR-containing plasmid were transfected at a molar ratio of 2:1:1 using PEI. ..

    Cell Culture:

    Article Title: SEMA7A orchestrates the colorectal cancer tumor microenvironment via PlexinC1: coordinating angiogenesis and macrophage M2 polarization.
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Article Title: Wnt5a-Ror2 signaling activates p62-Nrf2 axis in reactive astrocytes after brain injury
    Article Snippet: AAV-PHP.eB viruses were packaged in AAVpro 293T cells (Clontech). .. Briefly, AAVpro 293T cells were cultured in DMEM (FUJIFILM Wako Pure Chemical) supplemented with 10% (v/v) FBS (NICHIREI) and transfected with the pUCmini-iCAP-PHP.eB plasmid (Addgene), pHelper plasmid (Takara Bio) and AAV transfer plasmids using TransIT-VirusGEN Transfection reagent (Mirus). ..

    shRNA:

    Article Title: SEMA7A orchestrates the colorectal cancer tumor microenvironment via PlexinC1: coordinating angiogenesis and macrophage M2 polarization.
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Clarification Assay:

    Article Title: SEMA7A orchestrates the colorectal cancer tumor microenvironment via PlexinC1: coordinating angiogenesis and macrophage M2 polarization.
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Generated:

    Article Title: Retinal Epithelial Neutralization Assay Optimizes AAV Serotype Selection for Ocular Gene Therapy
    Article Snippet: .. Additionally, AAV vectors were generated via triple plasmid transfection of 293T cells, wherein the pHelper plasmid (Takara, Dalian, China, 6230), pRep-rCap plasmid (pRep-Cap2: Takara, Dalian, China, 6230; pRep-Cap5: HonorGene, Beijing, China, HG-VXH1155; pRep-Cap8: HonorGene, Beijing, China, HG-VXH1156; pRep-Cap9: HonorGene, Beijing, China, HG-VXH1157), and ITR-containing plasmid were transfected at a molar ratio of 2:1:1 using PEI. ..



    Similar Products

    86
    Cell Biolabs Inc phelper plasmid
    Phelper Plasmid, supplied by Cell Biolabs Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phelper+plasmid/phelper/10__1172_slash_jci200194-169-9-11
    Average 86 stars, based on 1 article reviews
    phelper plasmid - by Bioz Stars, 2026-10
    86/100 stars
      Buy from Supplier

    86
    Cell Biolabs Inc helper plasmid phelper
    Helper Plasmid Phelper, supplied by Cell Biolabs Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phelper+plasmid/phelper/pm42050331-228-28-31
    Average 86 stars, based on 1 article reviews
    helper plasmid phelper - by Bioz Stars, 2026-10
    86/100 stars
      Buy from Supplier

    94
    TaKaRa phelper plasmid
    Phelper Plasmid, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phelper+plasmid/AAVpro+Packaging+Plasmid/us12600969-701-1-6
    Average 94 stars, based on 1 article reviews
    phelper plasmid - by Bioz Stars, 2026-10
    94/100 stars
      Buy from Supplier

    96
    Addgene inc phelper
    Phelper, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phelper+plasmid/pAdDeltaF6+(Plasmid+%23112867)/pm41912662-343-24-25
    Average 96 stars, based on 1 article reviews
    phelper - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    86
    Cell Biolabs Inc adenoviral phelper plasmid
    Adenoviral Phelper Plasmid, supplied by Cell Biolabs Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phelper+plasmid/phelper/bio_rxiv__64898__2026__03__27__714707-321-6-9
    Average 86 stars, based on 1 article reviews
    adenoviral phelper plasmid - by Bioz Stars, 2026-10
    86/100 stars
      Buy from Supplier

    94
    Addgene inc aav helper plasmid phelper
    Aav Helper Plasmid Phelper, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phelper+plasmid/pAAV2%2F5+(Plasmid+%23104964)/bio_rxiv__64898__2026__02__27__708674-146-11-16
    Average 94 stars, based on 1 article reviews
    aav helper plasmid phelper - by Bioz Stars, 2026-10
    94/100 stars
      Buy from Supplier

    96
    Addgene inc phelper plasmid
    Phelper Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phelper+plasmid/pAdDeltaF6+(Plasmid+%23112867)/pmc12946740-61-0-3
    Average 96 stars, based on 1 article reviews
    phelper plasmid - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    Image Search Results